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HomePuzzles2181: Two-sided Strand Binder Design: Spherical 2

2181: Two-sided Strand Binder Design: Spherical 2

2181: Two-sided Strand Binder Design: Spherical 2

Standing: Energetic

Identify: 2181: Two-sided Strand Binder Design: Spherical 2
Standing: Energetic
Created: 08/03/2022
Factors: 100
Expires: 08/10/2022 – 23:00
Issue: Intermediate
Description: Design a beta strand and a separate protein that may bind to it! This puzzle is just like the earlier Spherical 1 puzzle, apart from a brand new Secondary Construction Goal that may penalize designs with >50% helices. We predict will probably be essential to design a binder that varieties a powerful beta sheet across the goal strand.

This can be a two-sided design downside, the place we are able to design the sequence of each binding companions. The aim is to design a pair of proteins that may self-associate in answer. That is particularly helpful for bioengineering purposes the place we would wish to use a brief peptide as a “sign” that may be acknowledged by its binding accomplice. Though the quick peptide could also be versatile in answer, we wish to bind it within the form of beta strand, so the goal spine has been frozen as a beta strand. Nevertheless, you should still change the sidechains of the strand goal to any amino acid you need!

AlphaFold predictions are enabled for the designed binder. As soon as you have designed a binder for the strand goal, add your answer for AlphaFold utilizing the AlphaFold prediction instrument. AlphaFold will predict the construction of your binder chain solely (i.e. within the unbound state, within the absence of the strand goal). In case you load this prediction, then Foldit will try and align the prediction together with your answer (i.e. within the sure state, making an interface with the strand goal). In case you proceed working off of the AlphaFold prediction, you might must make changes on the interface the place the binder interacts with the goal. See the puzzle feedback for extra Aims.

Classes: Design, General

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